Description
Description: This assay employs a two-site sandwich ELISA to quantitate ZC3H12C in samples. An antibody specific for ZC3H12C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ZC3H12C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZC3H12C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZC3H12C bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Northern blot analysis revealed that the expression of MCPIP1 and MCPIP3 was highly induced in macrophages in response to treatment with lipopolysaccharide (LPS). Although not affecting cell surface marker expression and phagocytotic function, overexpression of MCPIP1 significantly blunted LPS-induced inflammatory cytokine and NO production as well as their gene expression. Conversely, short interfering RNA-mediated reduction in MCPIP1 augmented LPS-induced inflammatory gene expression. Further studies demonstrated that MCPIP1 did not directly affect the mRNA stability of tumor necrosis factor alpha and monocyte chemoattractant protein 1 (MCP-1) but strongly inhibited LPS-induced tumor necrosis factor alpha and inducible nitric-oxide synthase promoter activation